Inactivation of microbial glutamin- (asparagin-) ase by azaserine and 6-diazo-5-oxo-L-norleucine
Lebedeva, Z.I.; Kabanova, E.A.; Berezov, T.T.
Biulleten' Eksperimental'noi Biologii i Meditsiny 100(12): 696-698
1985
ISSN/ISBN: 0365-9615 PMID: 4074870 Document Number: 258157
The effect of substrate analogues on glutamin-(asparagin-)ase from Pseudomonas aurantiaca-548 has been studied. The enzyme was demonstrated to be highly sensitive to the the action of 6-diazo-5-oxo-L-norleucine and azaserine. L-isomers of glutamine, aspartate, glutamate and several other substrate analogues with free alpha-amino groups protected the enzyme against the inhibitory DON effect. Thus, thorough preliminary selection of appropriate inhibitors, their dosage and treatment duration is needed for the recommendation of combined enzyme-inhibitor application in anti-tumour chemotherapy.