Comparison of non-histone proteins from hamster Kirkman-Robbins hepatoma and liver
Kiliańska, Z.; Kłyszejko-Stefanowicz, L.
Acta Biochimica Polonica 32(2): 95-100
1985
ISSN/ISBN: 0001-527X PMID: 4036452 Document Number: 257740
Three classes of non-histone proteins were obtained from hamster Kirkman-Robbins hepatoma and liver nuclei following separation of nucleic acids with the polyethylene glycol-dextran mixture and fractionation of nuclear proteins on hydroxylapatite in a salt-glycerol-phenylmethylsulphonyl fluoride system at increasing concentrations of Na+ and K+ phosphate buffer, pH 6.8. Two-dimensional polyacrylamide gel electrophoresis of these proteins documented their high heterogeneity; many spots were common but some spots specific only for neoplastic or normal tissue were also observed.