1-hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product
Jongeneelen, F.J.; Anzion, R.B.; Leijdekkers, C.M.; Bos, R.P.; Henderson, P.T.
International Archives of Occupational and Environmental Health 57(1): 47-55
1985
ISSN/ISBN: 0340-0131 PMID: 4077281 Document Number: 256621
A method for isolating 1-hydroxypyrene from urine is described. The presence of 1-hydroxypyrene in urine was identified by fluorescence excitation and emission scanning after HPLC-separation. 1-Hydroxypyrene could be detected in the urine of rats following oral administration of as little as 0.5 .mu.g pyrene. The dose-dependence of 1-hydroxypyrene in urine was evident after a wide range of pyrene dosing. After therapeutical coal tar treatment of dermatological patients the enhanced excretion of 1-hydroxypyrene was highly significant. Employees of a creosote impregnating plant showed an excretion pattern of 1-hydroxypyrene which could be related to their work. 1-Hydroxypyrene in urine of non-exposed people was very low, but detectable. It is suggested that the method reported is suitable for the assessment of uptake of man to pyrene, a compound that is commonly present in work environments which are associated with pollution of polycyclic aromatic hydrocarbons.