A tumor promoter enhances the phosphorylation of polyphosphoinositides while decreasing phosphatidylinositol labelling in lymphocytes
Boon, A.M.; Beresford, B.J.; Mellors, A.
Biochemical and Biophysical Research Communications 129(2): 431-438
1985
ISSN/ISBN: 0006-291X PMID: 2990456 Document Number: 254566
12-0-Tetradecanoyl-phorbol-13-acetate, a comitogen for lymphocytes, suppresses the concanavalin A-induced accumulation of 32P-phosphatidyl-inositol, in mouse spleen lymphocytes incubated with 32P-orthophosphate. The comitogenic tumor promoter does not affect the rate of de novo synthesis of phosphatidylinositol, as measured by [3H]-glycerol incorporation. Mitogen stimulates the incorporation of [32P]-phosphate into phosphatidylinositol, phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate, but the tumor promoter suppresses only the increased labeling of the phosphatidylinositol, while it enhances phosphorylation of the polyphosphionositides.