Assay of acenocoumarol (Sintrom) in the plasma by liquid chromatography

Guyon, F.; Dupeyron, J.P.; Fabiani, P.; Guyon, F; Dupeyron, J.P; Fabiani, P

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Assay of acenocoumarol (Sintrom) in the plasma by liquid chromatography

Guyon, F.; Dupeyron, J.P.; Fabiani, P.

Annales de Biologie Clinique 43(5): 743-745

1985

ISSN/ISBN: 0003-3898

PMID: 4091321

Document Number: 254560

Reversed-phase liquid chromatography was performed on C18 column using a pH 4 buffered water-methanol 45:55 (v/v) mobile phase and ultraviolet detection (307 nm). Warfarin was used as internal standard and dichloro-1,2 ethane as extraction solvent. The analytical recovery was greater than 98 p. cent. The within-day coefficient of variation (CV) was 5 p. cent, while the between day CV was 6.4 p. cent. The limit of detection was 0.02 mg/l.