Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes
Winter, E.; Radbruch, A.; Krawinkel, U.
EMBO Journal 4(11): 2861-2867
1985
ISSN/ISBN: 0261-4189 PMID: 2998759 Document Number: 254540
Four potentially productive and two non-productive VDJ gene segments were isolated from the DNA of mouse B-lymphocytes which had been polyclonally activated by bacterial lipopolysaccharide (LPS). Three VDJ regions exhibit VH genes which stem from two novel VH gene families. The complexity of these families is 5-9 genes. One of the non-productive VDJ regions exhibits a D segment which may have been generated by joining of two DSP2 segments. Both non-productive VDJ regions appear to contain rearranged pseudo VH genes. Three potential somatic mutations distributed over two productive VDJ regions are observed.