Induction of drug metabolizing enzymes in the liver of rats infested with Fasciola hepatica
Galtier, P.; Larrieu, G.; Lesca, P.
Journal of Pharmacy and Pharmacology 37(10): 751-754
1985
ISSN/ISBN: 0022-3573 PMID: 2867151 Document Number: 253692
Adult male rats were orally infected with 20 F. hepatica metacercariae. 5 weeks later they, and uninfected controls, were injected i.p. with either a single dose of Arochlor 1254 (200 mg/kg), 3-methylcholanthrene, beta -naphthaflavone (both 80 mg/kg) or 3 daily doses of phenobarbitone (80 mg/kg) and the liver enzymes were assayed 120, 48, 48 and 24 h resp. after the last injection. Fascioliasis induced decreases in aminopyrine N-demethylase and aniline hydroxylase concentrations in both control and all pre-treated rats, in cytochrome P-450 levels in controls, and phenobarbitone and Arochlor 1254 pretreated rats, and in cyclophosphamide mutagenic activity in phenobarbitone pretreated rats. The decreases may be due either to specific action of toxic excretions of the flukes or to the particular localization of tissue damage within the liver lobule. In contrast, plasma glutamic oxaloacetic transaminase, cytochrome b5, NADPH cytochrome c reductase, ethyoxycoumarin O-deethylase and the enzymatic activation of ethidium bromide were not affected by fascioliasis, irrespective of pretreatment.