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Identification of monoclonal antibodies against protein E1 of venezuelan equine encephalomyelitis virus blocking the hemagglutinating but not the infective activity of virions

Zhirnov, O.P.; Bukrinskaia, A.G.; Mel'nikova, E.E.; Gaĭdamovich, S.I.; Novokhatskiĭ, A.S.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 8: 31-35

1985

ISSN/ISBN: 0208-0613
PMID: 3842754
Document Number: 252527
Cellular clone (MAK 14-7), producing antibodies against the virus of Venezuela equine encephalomyelitis (VEE), strain 230, was isolated using the standard hybridomata technology. Monoclonal antibodies neutralized the viral hemagglutinating activity leaving the infectious one intact. Monoclonal antibodies from MAD 14-7 reacted specifically with viral glycoprotein E1 as registered by the immunoprecipitation technique. The topography of antigenic determinants of viral E1/E2 glycoprotein dimer forming the virions outer spikes is discussed in connection with the results obtained.

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