Specific immunoassay of alpha-amylase isoenzymes in human serum
Gerber, M.; Naujoks, K.; Lenz, H.; Gerhardt, W.; Wulff, K.
Clinical Chemistry 31(8): 1331-1334
1985
ISSN/ISBN: 0009-9147 PMID: 3893797 Document Number: 251925
A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.