Method of efficacious immunization against syngeneic murine sarcoma
Schaaf-Lafontaine, N.; Maquet, P.; De Graef-Lovens, D.; Courtoy, R.; Firket, H.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 179(1): 122-127
1985
ISSN/ISBN: 0037-9026 PMID: 3161594 Document Number: 251008
C57BL mice are immunized by subcutaneous injection of the T2 syngeneic tumour (MCA fibrosarcoma), followed a week later by surgical removal of the tumour. Such mice can be made hyper-immune by challenging them with mitomycin C-treated T2 cells and they do not develop a tumour after a subsequent inoculum of live T2 cells. Lymph nodes near the site of tumour inoculation are removed after 2 weeks and histological studies show an increase in the number of macrophages and mast cells but not of Lyt-2+ lymphocytes.