Nucleosidediphosphate-kinase induction by human recombinant IL-2 in mouse NK cells
Ohtsuki, K.; Yokoyama, M.; Ishii, F.; Ishida, N.
Biochemistry International 10(1): 13-21
1985
ISSN/ISBN: 0158-5231 PMID: 2985083 Document Number: 250238
The ability of human recombinant IL-2 to induce NDP-kinase in mouse NK cells has been studied. A significant increase in the amount of NDP-kinase was observed when the cells were exposed to IL-2 (100 units/ml) for 3 h at 37 degrees C. The enzyme inducting ability of human recombinant IL-2 was similar to that of native mouse IL-2 in the cells. The enzymatic characteristics [chemical requirements for the phosphoenzyme formation and molecular size of two distinct subunits (18,000 and 20,000 daltons)] of NDP-kinase from IL-2 treated cells were similar to those of the enzymes from EAT cells. The enzyme's biological role in the initiation of cell proliferation by IL-2 has been discussed.