A quantitative automated immunoassay for fibrinogen/fibrin degradation products
Schifreen, R.S.; Cembrowski, G.S.; Campbell, D.C.; Craig, A.R.; Demyanovich, N.D.; Jurga-Hall, P.A.; Reider, M.C.; Schwartz, M.W.; Tuhy, P.M.; Waller, S.J.
Clinical Chemistry 31(9): 1468-1473
1985
ISSN/ISBN: 0009-9147 PMID: 4028396 Document Number: 250122
We describe a prototype quantitative automated assay for fibrin and fibrinogen degradation products, a particle-enhanced turbidimetric inhibition immunoassay (PETINIA) in the Du Pont aca discrete clinical analyzer. This assay involves a latex particle reagent with covalently bound fibrinogen and a polyclonal antiserum raised in rabbits against human fibrinogen. A special secondary sample-collection tube quantitatively removes fibrinogen from citrated plasma and inhibits further fibrinolysis, independent of heparin concentration. The assay range is 0-100 mg/L, in fibrinogen equivalents. The CV for the assay is less than 10% when performed with the aca. Nonclottable fibrin and fibrinogen fragments are measured by the assay, the greatest sensitivity being directed at the E domain of the fibrinogen molecule. We illustrate with case studies the potential of this assay for providing clinical information not obtainable with currently available qualitative and semi-quantitative assays.