Extracellular distribution of radiolabel obscures specific binding of diethylstilbestrol in mouse skeletal muscle
Gruber, B.; Blix, P.M.; Cohen, L.
Research Communications in Chemical Pathology and Pharmacology 47(3): 445-448
1985
ISSN/ISBN: 0034-5164 PMID: 3992019 Document Number: 246938
The extracellular distribution of 3H-diethylstilbestrol (3H-DES) in mouse skeletal muscle was assessed following intraperitoneal injection. Total muscle extracellular space was measured with 14C-inulin, and the vascular space with 125I-albumin. A significant difference in the distribution of native 3H-DES and its metabolites in muscle and blood was found. This could only be explained if these compounds distributed with the albumin space and not the inulin space.