High-performance liquid chromatography of biotin and analogues
Chastain, J.L.; Bowers-Komro, D.M.; McCormick, D.B.
Journal of Chromatography 330(1): 153-158
1985
ISSN/ISBN: 0021-9673 PMID: 4044731 Document Number: 246243
Biotin, analogs and chemical intermediates were separated by high-performance liquid chromatography (HPLC) using reversed-phase and anion-exchange chromatographic conditions. Reversed-phase separations provided a wide range of retention times and resolution of nearly all the biotin compounds from mixtures of the analogs. Anion-exchange separations gave generally shorter retention times as compared to reversed-phase separations and greater resolution between biotin l- and d-sulfoxide. However, fewer analogs were resolved from mixtures of the compounds with anion-exchange HPLC.