Isoenzymes of mycobacteria. II. Relevance of LDH zymograms in taxonomy and identification
Katoch, V.M.; Sharma, V.D.; Datta, A.K.; Shivannavar, C.T.; Katoch, K.; Kannan, K.B.; Bharadwaj, V.P.
Indian Journal of Leprosy 57(1): 107-114
1985
ISSN/ISBN: 0254-9395 PMID: 3897403 Document Number: 244484
The cell free extracts of mycobacteria namely M. kansasii M. avium, M. tuberculosis, BCG (Glaxo), M. gastri, M. phlei, M. smegmatis, M. vaccae, M. strain w., M. scrofulaceum, M. gordonae, M. nonchromogenicum E. coli, Staph, aureus, and M. leprae infected skin have been electrophoresed and stained for LDH activity. Normal skin tissue was also taken as control. It was found that all the organisms tested showed distinct species specific LDH isoenzyme patterns. There was no extra band but an aberrant zone of LDH activity was seen in M. leprae infected human skin in comparison to LDH isoenzymes from normal skin. No strain variations was found among the different strains of species investigated. Results described in the present paper indicate that LDH isoenzyme patterns of mycobacteria could be of identification value at species level.