Experimental study upon platelet preservation by prostaglandin I2 during extracorporeal circulation
Osada, H.; Kawada, T.; Hoson, M.; Funaki, S.; Arase, K.; Masaki, H.; Taira, Y.; Kuwahara, M.; Noguchi, T.
Nihon Kyobu Geka Gakkai 32(10): 1795-1803
1984
ISSN/ISBN: 0369-4739 PMID: 6394659 Document Number: 240883
The hypothesis that prostaglandin I2 (PGI2) could preserve platelets during cardiopulmonary bypass by virtue of temporary reversible suppression of the platelet functions, especially of aggregability was tested. Twelve mongrel dogs were divided into 2 groups, the experimental (PGI2 treated) and the control, 6 for each. Cardiopulmonary bypass was carried out for 2 h by a roller pump through a sheet type bubble oxygenator in veno-arterial mode under normothermia. The PGI2 group received 100 mcg of PGI2 i.v. just prior to the onset of the perfusion, and 500 to 700 ng/kg/min of PGI2 drip-infused through the perfusion. Platelet aggregability to 20 .mu.M of ADP was seen suppressed down to .apprx. 11% of prebypass level during perfusion when checked immediately after sampling, but was restored to a level higher than that of the control group when an aliquot of sample left under room temperature for 60 min. More than 50% of prebypass aggregability was restored. Platelet count of the PGI2 group and the control group at 60 min of perfusion were 70.3% and 53.3% of prebypass level, respectively, the difference being statistically significant (P < 0.02). The PGI2 group kept higher level of platelet count at 90 and 120 min of perfusion, though less significant (P < 0.10). Four additional dog runs were done to see the behavior of platelet aggregability to varying dose of PGI2. With greater infusion rate of PGI2 (833 ng/kg per min) it was suppressed in excess, whereas with lesser rate (381 ng/kg/min on an average) it was paradoxically augmented and stayed so for > 1 h after sampling. PGI2 could well preserve platelets for 1 h during extracorporeal circulation through a bubble oxygenator in dogs, when given at rate 500-700 ng/kg/min.