Lithocholate detoxification and biliary secretion in the rat
Pattinson, N.R.; Chapman, B.A.
Biochemistry International 9(2): 137-142
1984
ISSN/ISBN: 0158-5231 PMID: 6487341 Document Number: 237749
To define the efficiency of hepatic detoxification, 14C lithocholate in combination with taurocholate was continuously infused intravenously into rats until steady-state. Quantitation of hepatic radiolabelled bile acid under these conditions showed only 11% of total liver bile acid was unmetabolised, indicating very efficient detoxification of lithocholate, in its most hepatotoxic state. Interestingly we found the rate for each bile acid to reach steady-state differed. To test whether the difference was due to glutathione S-transferase binding, as proposed by Strange et al (8), glutathione S-transferase levels were induced by phenobarbitone treatment. An increase in cytosol glutathione S-transferase levels had no effect on the time it took for each bile acid to reach steady-state.