Ta1, a novel 105 KD human T cell activation antigen defined by a monoclonal antibody
Fox, D.A.; Hussey, R.E.; Fitzgerald, K.A.; Acuto, O.; Poole, C.; Palley, L.; Daley, J.F.; Schlossman, S.F.; Reinherz, E.L.
Journal of Immunology 133(3): 1250-1256
1984
ISSN/ISBN: 0022-1767 PMID: 6205075 Document Number: 237284
By using a murine monoclonal antibody produced against an IL 2-dependent human T cell line, we defined a T lineage-specific molecule, termed Ta1, that is expressed strongly on activated T lymphocytes of both the T4 and T8 subsets, as well as on T cell lines and clones, but only weakly on a fraction of resting T cells. SDS-PAGE analysis of immunoprecipitates from 125I-labeled, activated T cells demonstrates a single major band of apparent m.w. 105 KD under both reducing and nonreducing conditions. Unlike anti-IL 2 receptor antibodies, anti-Ta1 does not inhibit T cell proliferative responses to mitogen, antigen, or IL 2-containing medium. Moreover, anti-Ta1 has no effect on T cell-mediated cytotoxicity. Ta1 appears to be a novel human T cell-specific activation antigen that may serve as a useful marker of T cell activation in human disease.