Host-cell reactivation of cis-diamminedichloroplatinum (II) -treated SV40 DNA in normal human, Fanconi anaemia and xeroderma pigmentosum fibroblasts
Poll, E.H.; Abrahams, P.J.; Arwert, F.; Eriksson, A.W.
Mutation Research 132(5-6): 181-187
1984
ISSN/ISBN: 0027-5107 PMID: 6096706 Document Number: 236956
Cell survival after treatment with cis-diamminedichloroplatinum(II)[cis-Pt(II)] and host-cell reactivation of cis-Pt(II)-treated SV40 DNA were investigated using 2 Fanconi anemia, 1 xeroderma pigmentosum of complementation group A and 3 normal human control fibroblast cell strains. The Fanconi anemia and xeroderma pigmentosum cell strains showed an increased sensitivity to the cytotoxic action of cis-Pt(II) treatment, suggesting a deficiency in the repair pathway of cis-Pt(II)-induced damage. The survival of cis-Pt(II)-treated SV40 DNA was about 2-fold lower in xeroderma pigmentosum cells than in control cells. No difference in viral DNA survival was found between Fanconi anemia and control cells, although the Fanconi anemia cells were more sensitive to the cytotoxic action of treatment with cis-Pt(II) than the xeroderma pigmentosum cells in the clonogenic cell survival assay.