Partial purification of extracellular toxic material of fish Vibrio
Moustafa, M.; Kodama, H.; Ishiguro, S.; Mikami, T.; Izawa, H.
American Journal of Veterinary Research 45(10): 2208-2210
1984
ISSN/ISBN: 0002-9645 PMID: 6497124 Document Number: 234881
The haemolytic activity of 3 pathogenic strains, of fish Vibrio commonly associated with vibriosis (V. anguillarum NCMB6 and NCMB571 strains, and Vibrio sp. N7802 strain) was examined, using chicken and mammalian erythrocytes. Vibrio strains NCMB6 and NCMB571 and their culture filtrates had haemolytic activity against 8 kinds of erythrocytes tested, whereas Vibrio strain N7802 produced only a small amount of haemolysin. Strain NCMB571 culture filtrate and its material partially purified by column chromatography were lethal in mice. From 2 peaks of protein, haemolysin was recovered from the 1st peak, which coincided with toxicity in mice. Heat-inactivation of culture filtrate indicated that haemolytic materials may be thermolabile proteins, but that the toxic material may be comparatively thermostable.