The mesothelial cell as a non-thrombogenic surface
Nicholson, L.J.; Clarke, J.M.; Pittilo, R.M.; Machin, S.J.; Woolf, N.
Thrombosis and Haemostasis 52(2): 102-104
1984
ISSN/ISBN: 0340-6245 PMID: 6523428 Document Number: 231217
A technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarly exposed to blood as a control were washed off the plastic.