Purine synthesis during amino acid starvation of lymphoblasts with HPRT deficiency or PP-ribose-P synthetase overactivity decreases less than in normal cells
Boss, G.R.
Advances in Experimental Medicine and Biology 165 Pt B: 15-19
1984
ISSN/ISBN: 0065-2598 PMID: 6326498 Document Number: 228059
Normal human lymphoblastoid cells starved for 3 hours for any of several essential but not nonessential amino acids decrease their rates of purine nucleotide synthesis via the de novo and salvage pathways by 90% and 60%, respectively (1). Cycloheximide and puromycin each reduced de novo synthesis by 96% and salvage synthesis by 72%. The decrease in purine synthesis de novo is of first order kinetics and in the case of amino acid deprivation is fully and rapidly reversible after reconsititution of the amino acid (1).