ELISA for IgG antibody to purified protein derivative (PPD) of patients with pulmonary tuberculosis
Koshino, T.; Nishioka, S.; Fujimura, M.; Okafuji, K.; Kanamori, K.; Mori, T.; Matsuda, T.; Kitao, T.; Kondo, K.; Hayashi, S.
Kekkaku 59(12): 621-624
1984
ISSN/ISBN: 0022-9776 PMID: 6335737 Document Number: 225572
An ELISA was developed for IgG antibody against purified protein derivative of tuberculin (PPD). PPD antibodies of 15 patients with active pulmonary tuberculosis and of 19 patients with inactive disease were measured at hospitalization. The control material consisted of 7 healthy blood donors and negative sera consisted of 7 healthy umbilical cord bloods. The mean antibody levels correlated with the tuberculosis infection. The mean antibody levels were significantly higher in the active tuberculosis patients than in the inactive patients. The mean antibody levels were higher in the active patients than in the controls. The mean antibody levels in the patients within 6 mo. after the discharge of bacilli ceased, were still as high as in the active patients. This assay was very simple and useful for diagnosis of the tuberculosis.