Lysosomal glycosidase activity in cultured human fibroblasts
Beliaeva, I.D.; Ivleva, T.S.; Vidershaĭn, G.I.
Biulleten' Eksperimental'noi Biologii i Meditsiny 98(11): 550-553
1984
ISSN/ISBN: 0365-9615 PMID: 6239662 Document Number: 224630
A study was made of the activity of 3 lysosomal glycosidases -beta-D-galactosidase (K. P. 3.2.1.23), alpha-L-fucosidase (K. P. 3.2.1.51), N-acetyl-beta-D-hexosoaminidase (K. P. 3.2.1.52) depending on the time after subcultivation and duration of the passage of human skin embryonal and postembryonal fibroblasts. It was established that changes in the specific activity of the enzymes should be calculated with reference to the cell rather than to protein whose amount might vary considerably. It was also found that for measuring the specific activity of enzymes, of great importance are the procedures of cell removal from the base layer (by mechanical scraping off or by trypsin solution) and the regimen of the homogenization of cell preparations.