Clinical application of immuno-precipitation inhibition technique for determination of disopyramide in human plasma
Nishino, T.; Minami, M.; Yoshida, I.; Shogase, T.; Sakurai, M.; Fujita, K.; Yasuda, H.; Yamada, K.; Togashi, H.; Saito, H.
Hokkaido Journal of Medical Science 59(4): 488-495
1984
ISSN/ISBN: 0367-6102 PMID: 6489920 Document Number: 224155
Immuno-precipitation Inhibition Technique (IPIT) method of determining disopyramide levels in human plasma was reevaluated in respect to factors affecting assay procedure, specificity and recovery. With this method only 20 microliters of plasma is required to obtain a rapid, sensitive and specific determination. Due to little intra-assay and inter-assay variation, the IPIT method is suitable for clinical determinations for disopyramide. Furthermore pharmacokinetic parameters calculated from the disopyramide concentrations coincided with the reported data determined by other methods. It was demonstrated, however, that precise temperature control and sample mixture are essential for accurate plasma disopyramide determination using this method.