Characterization of cell suspensions adhering to nylon obtained from mononuclear cells in toto or depleted of monocytes
Caruso, C.; Lio, D.; Modica, M.A.
Bollettino della Societa Italiana di Biologia Sperimentale 60(12): 2317-2323
1984
ISSN/ISBN: 0037-8771 PMID: 6335662 Document Number: 223169
The aim of this work was to evaluate the effect of monocyte depletion on the preparation of B cell enriched suspensions for DR typing. Nylon fiber adherent-cells obtained from total, or monocyte depleted, MNC were identified using different surface markers and cytochemical staining (SIg, DR, ANAE). The results demonstrate that no significant improvement in B enrichment is obtained by monocyte depletion.
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