Relation between PBPs (penicillin-binding proteins) and growth curves of Escherichia coli submitted to ampicillin and oxacillin
Yourassowsky, E.; Van der Linden, E.; Lismont, M.J.; Crokaert, F.
Pathologie-Biologie 32(5): 384-385
1984
ISSN/ISBN: 0369-8114 PMID: 6377206 Document Number: 222928
Growth curves recorded by the MS-2 (high performance photometer) provide a new means for studying PBPs. Indeed, the increase in prelytic OD (observed during the first two hours of contact between beta- lactams and cultures) is concentration- independent for antibiotics bound to PBPs3 ( Yourassowsky et al., 1981). Oxacillin has high affinity for PBP3 of E. coli (Curtis et al., 1979). Growth curves confirm biochemical studies: prelytic increase of OD of E. coli cultures is concentration- independent for oxacillin (100-2000 micrograms/ml), conversely to results observed with ampicillin and amoxycillin.