Conformational analysis of mixed monolayers of phorbol ester and phospholipid
Brasseur, R.; Deleers, M.; Ruysschaert, J.M.; Malaisse, W.J.
Biochemistry International 7(1): 71-78
1983
ISSN/ISBN: 0158-5231 PMID: 6689606 Document Number: 218774
A computational approach was used to study the conformation of mixed monolayers of amphiphilic molecules in order to characterize the assemblage of phospholipids around tumor-promoting and biologically inactive phorbol esters. The theoretical model was in fair agreement with both experimental data obtained in mixed monolayers of phorbol esters and dipalmitoylphosphatidylcholine spread at an air-water interface and the binding of phorbol esters to phospholipid bilayers. Thus, the present method may represent a useful tool to predict the orientation and molecular interaction of amphiphilic drugs at the membrane level.