Synergistic effects of human papillomavirus and phorbol ester in cell transformation
Cen, S.; Zhang, Y.; Xu, J.J.; Shen, Z.Y.; Zeng, Y.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 20(3): 260-262
2006
ISSN/ISBN: 1003-9279 PMID: 17086288 Document Number: 599539
To investigate the molecular mechanism of the synergistic effects of human papillomavirus (HPV) and phorbal esters (TPA) in cell transformation. The expression of oncogenes and anti-oncogenes in 293 cell line treated with HPV and TPA was studied by Southern Blot and RNA dot blot. It was found that the synergistic effect induced the amplification of c-myc (4-8 times), increased expressing level of c-erbB-2 (32-64 times) and decreased expressing level of p16(1/4-1/8). The above results show that the synergistic effect has an important role in development of carcinoma.