Effect of 5 alpha-androstane-3 beta,17 beta-diol on estradiol-17 beta reception in pituitary cytosol of male and female rats
Degtiar', V.G.
Biokhimiia 48(9): 1479-1482
1983
ISSN/ISBN: 0320-9725 PMID: 6626608 Document Number: 218478
The strongest inhibiting effect of estradiol-17 beta (E2) reception in the cytosol fraction of male and female rat pituitary is exerted by 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol). Sucrose density linear gradient centrifugation of pituitary cytosol preincubated with [3H]E2 in the presence of 3 beta-diol demonstrated that in the pituitary cytosol of rats of both sexes 3 beta-diol affects the non-specific binding of [3H]E2 around 4.5 S, when taken both at high and at low concentrations. The specific binding of [3H]E2 at 8.0-8.5 S is inhibited only by high concentrations of 3 beta-diol. It is assumed that 3 beta-diol can take part in the regulation of E2 reception in male and female rat hypophyses.