Method of isolation of intact enterocytes from rat duodenal mucosa
Sukhomlinov, B.F.; Fedorovich, A.N.; Chaĭka, I.P.
Ukrainskii Biokhimicheskii Zhurnal 55(2): 199-201
1983
ISSN/ISBN: 0201-8470 PMID: 6845446 Document Number: 215389
A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.
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