Detection of antibody to epizootic diarrhea of infant mice (EDIM) virus
Smith, A.L.; Knudson, D.L.; Sheridan, J.F.; Paturzo, F.X.
Laboratory Animal Science 33(5): 442-445
1983
ISSN/ISBN: 0023-6764 PMID: 6316027 Document Number: 214857
Cultured mouse liver cells containing either epizootic diarrhoea of infant mice viral antigen or Nebraska calf diarrhoea vaccine virus antigen were equally effective for detecting serum antibody to epizootic diarrhoea of infant mice virus by immunofluorescence. The presence of rotavirus in infant mouse intestinal suspensions used as cell culture inocula was confirmed by polyacrylamide gel electrophoresis of the double-stranded RNA viral genome. In a limited comparison using simian rotavirus SA 11 viral antigen, the fluorescence test was specific but less sensitive than an enzyme-linked immunosorbent assay.