Characterization of an antigen expressed by human natural killer cells
Griffin, J.D.; Hercend, T.; Beveridge, R.; Schlossman, S.F.
Journal of Immunology 130(6): 2947-2951
1983
ISSN/ISBN: 0022-1767 PMID: 6574190 Document Number: 211537
A monoclonal antibody, anti-N901, was produced by fusing NS-1 cells. Monocytes, granulocytes, B cells, T cells (T3+ cells), erythrocytes and platelets were nonreactive. The N901-positive cells in the peripheral blood were heterogeneous with respect to expression of other cell surface antigens. The majority of N901+ cells co-expressed T11, Mo1 and HNK-1; a smaller percentage expressed T8.Ia, T3, T4, Mo2 or B1 antigens were very uncommon on N901+ cells. The heterogeneity of the N901+ LGL was further investigated by examining the expression of N901 antigen on a series of cloned normal human NK cell lines. N901 antigen was expressed by each of the NK cell lines tested and by a minority of cloned T cell lines without NK activity. Anti-N901 does not block NK activity and can be used to rapidly purify functional NK cells for further study.