Multisite study of cryptosporidiosis in children with diarrhea in India
Ajjampur, S.S.R.; Liakath, F.B.; Kannan, A.; Rajendran, P.; Sarkar, R.; Moses, P.D.; Simon, A.; Agarwal, I.; Mathew, A.; O'Connor, R.; Ward, H.; Kang, G.
Journal of Clinical Microbiology 48(6): 2075-2081
2010
ISSN/ISBN: 1098-660X PMID: 20392919 DOI: 10.1128/jcm.02509-09Document Number: 211081
Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.