Comparison of pyrimidine 5'nucleotidase activity in erythrocytes of sheep, dogs, cats, horses, calves, and Mongolian gerbils
George, J.W.; Duncan, J.R.; Mahaffey, E.A.
American Journal of Veterinary Research 44(10): 1968-1970
1983
ISSN/ISBN: 0002-9645 PMID: 6314858 Document Number: 207805
Pyrimidine 5'nucleotidase (P5N) activities of erythrocytes for Mongolian gerbils, cats, dogs, sheep, horses, and calves were measured, using a radiometric technique with [14C]cytidine monophosphate as the substrate. Erythrocytes of gerbils had the highest activity [1177 +or- 133 mU/g of haemoglobin (Hb)]. Feline erythrocytes had 327 +or- 204 mU/g of Hb. Canine erythrocytes had 148 +or- 20 mU/g of Hb. Ovine erythrocytes (44.3 +or- 20.9 mU/g of Hb), equine erythrocytes (30 +or- 16 mU/g of Hb), and bovine erythrocytes (14 +or- 7) had relatively low P5N activity. The P5N activity was approximately proportional to the proportion of reticulocytes and inversely proportional to the mean erythrocyte life span in these species.