Ultrastructural expression of the process of cell fusion in a rat neurinoma culture exposed to polyethylene glycol
Poliakova, G.P.; Chudinovskaia, N.V.; Kondakova, L.I.
Biulleten' Eksperimental'noi Biologii i Meditsiny 96(9): 114-117
1983
ISSN/ISBN: 0365-9615 PMID: 6616042 Document Number: 207646
The time course of cell fusion was examined in experiments made with a new cell line derived from rat neurinoma. Exposure to polyethylene glycol (PEG) combined with dimethylsulfoxide led to absorption of cells on plasmalemmas, modification of glycocalyx, disorganization of plasmalemmas in local zones of adjacent membranes, and formation of the common zones of the cytoplasm between neighboring cells. The method used promoted the obtaining of polykaryons, which was accompanied by a decrease in PEG cytotoxicity.