Use of polyethylene glycol (PEG) in the isolation of the IBR virus
Ignatov, G.; Khristova, V.
Veterinarno-Meditsinski Nauki 13(4): 73-76
1976
ISSN/ISBN: 0324-1068 PMID: 183343 Document Number: 98683
Experiments were carried out to use polyethylene-glycol (PEG)--6000 in the isolation of the IBR virus from field material. The sedimentation with PEG proved easy, fast and selective. Large amounts of field material suspension could be treated which made it possible to attain volume concentration of such suspensions and to raise the relative concentration of the virus in them. Thus the virus could be isolated more quickly and in a more dependable way, avoiding the toxic effect of some of the initial materials on the cell culture.