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Use of the keratoconjunctival test for determining the virulence of the causative agent of pseudotuberculosis

Polotskiĭ, I.E.; Tseneva, G.I.; Efremov, V.E.; Kleganov, V.K.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 72-76

1983

ISSN/ISBN: 0372-9311
PMID: 6346760
Document Number: 207125
When tested in guinea pigs, Y. pseudotuberculosis strains, serovar I (25 strains) and serovar V (1 strain), were found for the first time to be capable of causing keratoconjunctivitis in the animals; the most virulent of these strains caused progressive conjunctivitis and keratitis with generalized infection. The minimum infective dose was 10(4) for conjunctivitis and 10(6) for keratitis. The studied Y. pseudotuberculosis strains, serovars O3 and O9, were found to be incapable of causing conjunctivitis and keratitis. The authors believe that the keratoconjunctival test allows one to evaluate the invasiveness and toxicity of Y. pseudotuberculosis according to the degree of the manifestation and the time of the development of conjunctivitis and keratitis.

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