Fluorescence microscopic study of cells and polykaryons in the early periods following polyethylene glycol treatment
Borodina, V.M.; Kir'ianova, E.A.; Zelenin, A.V.
Tsitologiia 25(7): 759-764
1983
ISSN/ISBN: 0041-3771 PMID: 6623635 Document Number: 203820
A fluorescence-microscopical study is made of cultured murine fibroblasts (L-cells) in early periods after the treatment with polyethylene glycol (PEG). Optimal conditions of fusion procedure were found under which the effectiveness of fusion was the highest and the toxical effect of PEG the lowest. The number of dead cells after the treatment with PEG did not exceed 10%. No significant changes in chromatin cytochemical properties (Acridine Orange and Olivomycin binding) were observed in the early periods of PEG treatment, that allows to use PEG for studying chromatin properties in hybrid cells obtained by PEG fusion. By means of PEG fusion, the hybrid cells with prematurely condensed chromosomes and also hybrids between animal and yeast cells have been obtained.