The inhibition of the mitogenic stimulation of B lymphocytes by a serine protease inhibitor: commitment to proliferation correlates with an enhanced expression of a cell-associated arginine-specific serine enzyme
Ku, G.S.; Quigley, J.P.; Sultzer, B.M.
Journal of Immunology 131(5): 2494-2499
1983
ISSN/ISBN: 0022-1767 PMID: 6355295 Document Number: 199888
Using diisopropylfluorophosphate (DFP) it was shown that the mitogenic stimulation of murine B lymphocytes can be maximally inhibited a few hours before commitment of the cells to DNA synthesis. This inhibition was shown to be specific for a serine enzyme(s). The results of experiments designed to determine the location of this enzyme indicated that the mitogens-linked serine enzyme is not a secreted extracellular factor but is cell-associated. Fluorographic analysis of electrophoretic gels of cell homogenates labeled with MW) suggesting that this protein may be critical to the progression of the cells through the cell cycle into the S phase of DNA synthesis.