Identification of macrophages and of a T-lymphocytes subpopulation in normal human lymphnode by histochemical demonstration of alpha-naphthyl-acetate-esterasic activity
Manara, G.C.; Scandroglio, R.; De Panfilis, G.; Allegra, F.
Acta Bio-Medica de l'Ateneo Parmense 54(3): 237-245
1983
ISSN/ISBN: 0392-4203 PMID: 6228089 Document Number: 197394
The cell population of normal human lymphnode was studied by a histochemical technique showing alpha-naphthyl-acetate-esterasic activity. Two distinct cell populations were evidenced: a) macrophagic cells, bearing a diffused ANAE cytoplasmic positivity, most of which were in the subcapsular area, whereas a minority of them was found within the lymphatic nodules and the paracortical area; b) lymphocytic cells, bearing on ANAE positive cytoplasmic spot, most of which were in the paracortical area, whereas a minority of them was found within the lymphatic nodules. A discussion of the results we got, as compared with those which are reported by literature, confirms what is already well-known about the cell colonization of the various areas in the lymphnode. Besides it gives a chance to identify lymphocytes bearing an ANAE positive cytoplasmic spot with T lymphocytes belonging to the "helper-inducer" subclass.