Procedure for blood glutathione peroxidase determination in cattle and swine
Agergaard, N.; Jensen, P.T.
Acta Veterinaria Scandinavica 23(4): 515-527
1982
ISSN/ISBN: 0044-605X PMID: 7168428 Document Number: 195938
An improved testing system was developed for direct measurement of glutathione peroxidase (GSH-Px) activity in heparinized whole blood at 37 degrees C. Without loss in net yield, the consumption of reagents was considerably lower with the new technique than with previously described techniques. Within the range 0-700 mKat/litre, GSH-Px activity in red cells was estimated with a high degree of accuracy and reproducibility without previous separation and washing. The stability of the enzyme in bovine and porcine whole blood at 22 degrees , 4 degrees and -20 degrees C was estimated. An improved testing system was developed for direct measurement of glutathione peroxidase activity in heparinized whole blood at 37 deg C. Without loss in net yield the consumption of reagents was considerably lower with the new technique than with previously described techniques. Within the range 0-700 mKat/l the GSH-Px activity in red cells may be measured with a high degree of accuracy and reproducibility without preceding separation and washing. The stability of the enzyme in bovine and porcine whole blood at 22 deg C, 4 deg C, and 20 deg C was determined.