Simple, reliable chromatographic measurement of oxalate in urine
Di Corcia, A.; Samperi, R.; Vinci, G.; D'Ascenzo, G.
Clinical Chemistry 28(7): 1457-1460
1982
ISSN/ISBN: 0009-9147 PMID: 7083555 Document Number: 194422
In this assay for oxalate in urine, oxalate is absorbed from the urine onto graphitized carbon black (Carbopack B). After desorption and removal of the solvent, oxalic acid is gas-chromatographically measured after being derivatized with BF3/methanol. Chromatography is on Carbopack B/polyethylene glycol (Mr gave a mean of 47.1 (SD 15.4) mg/24 h, with an analytical recovery of 97.4% (SD 3.0%, range 93.6-102.4%). Total analysis time for 1 sample is .apprx. 2 h.