Prostaglandin F2 alpha is associated with alveolar bone cells: immunohistochemical evidence utilizing monoclonal antibodies
Davidovitch, Z.; Shanfeld, J.L.; Lally, E.
Progress in Clinical and Biological Research 101: 125-134
1982
ISSN/ISBN: 0361-7742 PMID: 6760204 Document Number: 192635
Prostaglandin F2 alpha (PGF2 alpha) has been found in mineralized tissues and may be implicated in cellular remodeling activities. The objective of this study was to localize PGF2 alpha in cat jaw bone by immunohistochemistry. Monoclonal antibodies to PGF2 alpha were prepared by the hybridoma technique and used as the first step in the tissue section staining procedure, the rabbit anti-mouse IgG conjugated to horseradish peroxidase followed by diaminobenzidine (DAB) as the substrate. Periodontal cells and cementoblasts displayed dark DAB deposits over the cell periphery. Some alveolar bone osteoblasts and osteoclasts were stained for PGF2 alpha over the cytoplasm as well as the membrane region. In the maxillary-premaxillary suture, osteoblasts on the border of the maxilla were stained very intensely for PGF2 alpha, while those adjacent to the premaxilla were stained exceptionally lightly. These results demonstrate the ability to localize PGF2 alpha in bone cells in situ and to identify intracellular sites of prostaglandin localization. Our observation suggests that PGF2 alpha may play a role in physiologic activities of fibroblasts, osteoblasts, and osteoclasts.