Role of CO-binding cytochrome c in enzymatic oxidation of methane by the bacterium Methylococcus capsulatus

Gvozdev, R.I.; Nikonova, E.L.; Piliashenko-Novokhatnyi, A.I.; Shushenacheva, E.V.; Grigorian, A.N.

Biokhimiia 47(7): 1118-1124

1982


ISSN/ISBN: 0320-9725
PMID: 6288124
Document Number: 192139
The cytochrome c spectrally related to cco cytochromes has been isolated and purified from the methane-oxidizing bacterium Methylococcus capsulatus. The cytochrome binds CO but does not bind other substrates of methane monooxygenase, does not activate the methane monooxygenase reaction and is not a component of methane monooxygenase. In the methanol dehydrogenase enzymatic system cytochrome cco functions as electron acceptor. A possible role of cytochrome cco as electron carrier intermediate in the sequence of the dehydrogenase and oxidase enzymatic systems of M. capsulatus is discussed.

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