Critical comments on the calculation of receptor analyses
Kuss, E.
Journal of Clinical Chemistry and Clinical Biochemistry 20(4): 227-234
1982
ISSN/ISBN: 0340-076X PMID: 7077239 Document Number: 191536
The main literature concerning the processing of binding data is reviewed. Practical conclusions for the measurement of association constants and binding site concentrations are considered from the standpoint of basic binding theory in connection with basic information theory. The theoretical minimum errors in the association constant and the binding site concentration are discussed as a function of the saturation fraction of the most dilute component. Various plotting forms and the error arising from various rearrangements of the basic binding equation are discussed. For fitting procedure several heuristic models are mentioned and it is recommended to start from the most general Adair function. The necessity of well defined criteria for quality of fit in computer programming for iterative curve fitting procedures is emphasized. The most frequent, however avoidable, errors are mentioned.