Titrimetric and gravimetric calibration of pipettors: a survey

Ryan, W.

American Journal of Medical Technology 48(9): 763-766

1982


ISSN/ISBN: 0002-9335
PMID: 7137214
Document Number: 190977
Methods for gravimetric and titrimetric calibration of pipets are described. Both methods of calibration gave good reproducibility from 5-500 microliters. The titrimetric method gave the lowest coefficient of variation for pipettors from 1-10 microliters and was the better method for these smaller volumes. In addition, the titrimetric method took one-third the time of the gravimetric method. Pipettors of various types were obtained from 38 different laboratories and used to survey performance. The titrimetric method was used to calibrate 572 pipettors of different types. A significant percentage of the pipettors were greater than 3% from their nominal value.

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