Studies on influence of detergents on human serum albumin using temperature-perturbation difference spectrophotometry

Zarechnaia, I.P.; Bagdasar'ian, S.N.; Troitskiĭ, G.V.; Krivoshein, I.S.; Rud'ko, A.P.

Ukrainskii Biokhimicheskii Zhurnal 54(4): 383-388

1982


ISSN/ISBN: 0201-8470
PMID: 7135495
Document Number: 189061
The temperature-perturbation difference spectra (TPDS) of human serum albumin (NSA) were studied after incubation with different synthetic detergents--anionic, cationic, amphoteric, nonionogenic--in various molar ratios. It is shown that the detergents studied have a different effect on the perturbation of tyrosine residua spectra. Under these conditions the rigidity of their environment depending on the nature and concentration of the mentioned detergents is supposed to change.

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