Turnover rates of cat skeletal muscle soluble enzymes
Inestrosa, N.C.; Fernandez, H.L.
Cellular and Molecular Biology 28(6): 541-545
1982
ISSN/ISBN: 0145-5680 PMID: 6820640 Document Number: 188250
Turnover rates of cat geniohyoid muscle glycolytic and glycogenolytic enzymes were studied after local application of 10 mM colchicine into the hypoglossal nerve. Drug treatment caused reversible decreases in the activities of malic dehydrogenase, pyruvic kinase and phosphorylase-b, which have been previously ascribed to a temporary interruption of axonal transport. Kinetics of the return of the enzymic activities to normal steady-state levels were analyzed to calculate half-lives of 16 days for malic dehydrogenase, 14 days for pyruvic kinase and 21 days for phosphorylase-b. The turnover rate of certain muscle soluble enzymes is partly dependent on the subcellular localization where the enzymes play their physiological role.