Blood volume of nonsplenectomized and splenectomized cats before and after acute hemorrhage
Breznock, E.M.; Strack, D.
American Journal of Veterinary Research 43(10): 1811-1814
1982
ISSN/ISBN: 0002-9645 PMID: 7149382 Document Number: 187661
Blood volume (BV) was determined in awake, nonsplenectomized (NSPX) and splenectomized (SPX) cats before and after hemorrhage (6 ml/kg). Each NSPX cat had a determined BV at least 10 ml/kg greater than the same cat after splenectomy. The mean BV of SPX cats was 43.4 .+-. 8.94 ml kg (4.3% of body wt). The calculated RBC masses of NSPX and SPX cats were 17.0 .+-. 4.07 and 12.2 .+-. 1.12 ml/kg, respectively. Each NSPX cat had apparent RBC masses of 5 ml/kg greater than that of the same cat after splenectomy was done. At 1 h after a hemorrhage, the BV and RBC masses determined in SPX cats were 46.7 .+-. 12.1 and 9.7 .+-. 1.90 ml/kg, respectively. Extravascular-to-intravascular fluid flux (calculated from RBC masses and plasma protein dilution) was approximately 0.80% of body wt. The indirect method with 51Cr-labeled RBC for BV determination was accurate and precise in awake, SPX cats; in awake, NSPX cats, the 51Cr-labeled RBC dilution method was precise, but not accurate. The spleen in the cat resulted in marked overestimations of BV and RBC masses.